SSC 20X buffer

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in situ recipes

20x SSC 5 ml 20 ml dH2O 995 ml 3960 ml Tween 20 5 ml 20 ml Proteinase K / RNASE Buffer Stock 1000 ml 4000 ml 500 mM NaCl 5 M 100 ml 400 ml 10mM Tris pH 8.0 1 M 10 ml 40 ml dH2O 890 ml 3560 ml For prehyb PK buffer - use DEPC or ddH20 treated water to make solution.For posthyb RNase buffer,use regular dH2Oin situ recipes20x SSC 5 ml 20 ml dH2O 995 ml 3960 ml Tween 20 5 ml 20 ml Proteinase K / RNASE Buffer Stock 1000 ml 4000 ml 500 mM NaCl 5 M 100 ml 400 ml 10mM Tris pH 8.0 1 M 10 ml 40 ml dH2O 890 ml 3560 ml For prehyb PK buffer - use DEPC or ddH20 treated water to make solution.For posthyb RNase buffer,use regular dH2OUltraPure SSC,20XUltraPure 20X SSC is a solution formulated for use in nucleic acid hybridizations and blot transfer applications.It is used in concentrations ranging from 0.2X to 20X,depending on the application.Supplied in 1 L plastic bottles,or in a 4 L or 10 L stackable Cubitainer Box,sterile-filtered Ultra

Ssc Buffer (20x) at Thomas Scientific

20X SSC buffer is a solution formulated for use in nucleic acid preparation and blotting applications,including northern blotting.It is used in concentrations ranging from 0.2X to 20XSsc Buffer (20x) at Thomas Scientific20X SSC buffer is a solution formulated for use in nucleic acid preparation and blotting applications,including northern blotting.It is used in concentrations ranging from 0.2X to 20XSodium Citrate Buffer at Thomas Scientificbuffer with sodium perborate is use as a substrate buffer in immunoassay systems utilizing the enzyme horseradish peroxidase.Reconstitution Empty the contents of one capsule in 100 mL of deionized water and dissolve.The contents of one capsule yields 0.05 M phosphate-citrate buffer

Saline-Sodium Citrate (SSC),20X Solution (Molecular

SalineSodium Citrate (SSC) is used as a buffer in Southern transfer protocols.This product(s) resides on a Fisher Scientific GSA or VA contract.If you are viewing this page as a nonregistered user,the price(s) displayed is List Price.SSC,20X Buffer,Quality Biological VWRSSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridizationThe electrophoresis buffers maintain consistent pH levels during DNA,RNA,and other protein separation.Providing ions that transfer the applied current,the low charge solutions facilitate different molecular sizes to travel SSC,20X Buffer,Quality Biological VWRSSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridizationThe electrophoresis buffers maintain consistent pH levels during DNA,RNA,and other protein separation.Providing ions that transfer the applied current,the low charge solutions facilitate different molecular sizes to travel

SSC buffer (20X) - J60839 - Alfa Aesar

SSC buffer (20X) MDL.MFCD00132371.Propri SSC 20X buffer#233;t SSC 20X buffer#233;s chimiques.Forme.Liquid.Storage Sensitivity.Ambient temperatures.Solubilit SSC 20X buffer#233;.Fully misicible in water.Applications.SSC buffer is suitable for preparing nucleic acids for hybridization,hybridization buffer for northern and southern blots,washing buffer for northern and southern blots SSC buffer (20X) - J60839 - Alfa AesarJ60839 SSC buffer (20X) CAS Number.Synonyms SDS Certificate of Analysis Product Specification Technical Inquiry Stock No.Size Price ($) Quantity Availability; J60839-K2 1L 29.20 J60839-K3 2L 51.40 J60839-K7 4L 94.00 Add to Cart Bulk/Specialty Print Quote View Item SSC buffer (20X) (3 M,pH 7) Preparation and Recipe AAT SSC buffer (20X) (3 M,pH 7) preparation guide and recipe.Recipe can be automatically scaled by entering desired final volume.Saline-sodium citrate (SSC) buffer is often used as a hybridization buffer for DNA and RNA,particularly for Northern and Southern blots.

SSC Buffer,Concentrate,OmniPur*.20X.VWR

SSC Buffer,Concentrate,OmniPur*.20X.Buffers Electrophoresis Buffers Hybridization Buffers.Liquid.Each liter of concentrate makes 20L of 1X SSC (0.15M NaCl,0.15M sodium citrate).DNase-,RNase-,and protease-free.Order Now.ORDER; RELATED INFORMATION; Q and A; Enter Certificate Details SSC Buffer,20X,Molecular Grade - PromegaSSC Buffer,20X,Molecular Grade (pH 7.0),is commonly used in nucleic acid hybridization techniques at concentrations from 0.1X to 20X,depending on the application.Form Clear,colorless liquid.Composition 3M NaCl,0.3M sodium citrate (for 20X concentration).SSC Buffer 20 SSC 20X buffer#215; Concentrate for Northern and Southern SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures.It is used to control stringency of wash buffer for the washing steps after hybridization.Application SSC Buffer 20 SSC 20X buffer#215; Concentrate has been used as a

SSC Buffer (20X) for Molecular Biology - GeneON-BioScience

SSC Buffer (20X) For preparing nucleic acids for hybridization and Northern and Southern blots.Washing buffer for Northern and Southern blots (Molecular Biology Grade) State of matter Liquid additional product.description.20X concentrated aqueous solution SSC Buffer (20X) National DiagnosticsSSC Buffer (20X) is a concentrated solution of sodium chloride-sodium citrate in distilled/deionized water.SSC Buffer is widely used in nucleic acid blotting and hybridization protocols.SSC Buffer (20X) is already in solution,which eliminates the weighing,mixing,and adjusting ofSSC Buffer (20X) - genemedFast Delivery Orders are normally shipped within 24 hours from the time of the order receipt.Orders placed before 1PM PST are normally shipped the same day.

SSC BUFFER,20X CONCENTRATE - americanbio

SSC BUFFER,20X CONCENTRATE Safety Data Sheet according to Federal Register / Vol.77,No.58 / Monday,March 26,2012 / Rules and Regulations 07/22/2014 EN (English US) 2/6 4.3.Indication of any immediate medical attention and special treatment needed No additional information available SECTION 5 Firefighting measures 5.1.SSC 20X buffer - eLABProtocolsVolume of 20X SSC buffer Step 1.Prepare 20X SSC buffer by adding NaCl (3M) Sodium citrate (0.3M) Step 2.Dissolve in ddH 2 0 and adjust the pH to 7.2.Step 3.Add ddH 2 O to a total volume of and autoclave Attachments.no file attachments.This procedure was originally created by Admin eLABJournalSSC 20X BUFFER (fr)20xSSC solution for use in Southern and northern blotting.

SSC - CSH Protocols

SSC.For a 20X solution Dissolve 175.3 g of NaCl and 88.2 g of sodium citrate in 800 mL of H 2 O.Adjust the pH to 7.0 with a few drops of a 14 N solution of HCl.Adjust the volume to 1 L with H 2 O.Dispense into aliquots.Sterilize by autoclaving.SSC - CSH ProtocolsSSC.For a 20X solution Dissolve 175.3 g of NaCl and 88.2 g of sodium citrate in 800 mL of H 2 O.Adjust the pH to 7.0 with a few drops of a 14 N solution of HCl.Adjust the volume to 1 L with H 2 O.Dispense into aliquots.Sterilize by autoclaving.SSC (20X),RNase-freeMolecular biology grade,Ambion 20X SSC solution is supplied in four bottles containing 1 L each.The buffer is certified RNase-free,economical,and ready-to-use.Due to the ubiquitous presence of RNases,manufacturing products for use with RNA is especially challenging.Ambion's nuclease-free reag

SAFETY DATA SHEET - Fisher Scientific

SAFETY DATA SHEET Revision Date 18-Jan-2018 Revision Number 3 1.Identification Product Name Saline Sodium Citrate 20X Solution Cat No. BP1325-1; BP1325-4; BP1325-20 Synonyms SSC buffer; Sodium Citrate-Saline mixture Recommended Use Laboratory chemicals.Uses advised against Food,drug,pesticide or biocidal product use.Details of the supplier of the safety data sheetRelated searches for SSC 20X buffer20x ssc buffer recipe20x ssc buffer preparationssc bufferssc buffer recipeSome results are removed in response to a notice of local law requirement.For more information,please see here.12345NextSSC buffer (20X) - J60839 - Alfa AesarJ60839 SSC buffer (20X) CAS Number.Synonyms SDS Certificate of Analysis Product Specification Technical Inquiry Stock No.Size Price ($) Quantity Availability; J60839-K2 1L 29.20 J60839-K3 2L 51.40 J60839-K7 4L 94.00 Add to Cart Bulk/Specialty Print Quote View Item Related searches for SSC 20X buffer20x ssc buffer recipe20x ssc buffer preparationssc bufferssc buffer recipePrevious123456NextSaline-Sodium Citrate (SSC),20X Solution (Molecular SalineSodium Citrate (SSC) is used as a buffer in Southern transfer protocols.This product(s) resides on a Fisher Scientific GSA or VA contract.If you are viewing this page as a nonregistered user,the price(s) displayed is List Price.

Related searches for SSC 20X buffer

20x ssc buffer recipe20x ssc buffer preparationssc bufferssc buffer recipePeople also askWhat is buffer 20x?What is buffer 20x?More storage temp.SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures.It is used to control stringency of wash buffer for the washing steps after hybridization.SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).SSC Buffer 20 SSC 20X buffer#215; Concentrate for Northern and Southern blotting,solutioOxford Laboratory Technology How to make 1x buffer from 20xMay 17,2014 SSC 20X buffer#0183;How to make 1x buffer from 20x As explained before here it is very simple and you need to understand the concept.or you can use the formula M1V1=M2V2 Example You have 20x Orange Juice and you wanna make 10 ml of 1x.Simple use M1V1=M2V2 M1= Original Concentration [which is 20x]

In situ hybridization - Xenbase

5x SP6 Buffer MEMFA PTw Triethanolamine 0.1 M PTw Containing 4% Paraformaldehyde Hybridization Buffer SSC,20X Denhardts solution,100X RNase A RNase T1 4% BMB Blocking Reagent in MAB Alkaline Phosphatase Buffer Levamisol 1 M NBT/BCIP PVA Alkaline Phosphatase Buffer Bouins Fixative Bleaching solution (Mayor et al.1995) Nucleotide StocksImages of SSC 20X Buffer imagesSSC Buffer,20X Concentrate Reagent designed and SSC Buffer,20X Concentrate,is a standard reagent in Southern and Northern blotting procedures.This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5X.It is also for use in preparing nucleic acids for hybridization.Physical form20x SSC Solution 20x SSC SolutionThe 20x SSC Solution must be stored at RT.If these storage conditions are followed,the 20x SSC Solution will function,without loss of performance,at least until the expiry date printed on the label.55..5.Instruction .Instruction .The 20x SSC Solution is a 20x concentrate which needs to be diluted

20x SSC Solution 20x SSC Solution

The 20x SSC Solution must be stored at RT.If these storage conditions are followed,the 20x SSC Solution will function,without loss of performance,at least until the expiry date printed on the label.55..5.Instruction .Instruction .The 20x SSC Solution is a 20x concentrate which needs to be diluted20x SSC Buffer - Cytocell20x SSC Buffer.Applications ancillary items.Catalogue Numbers PCA 003.Request a quote.Request a sample.Unit Size 100ml.Browse all Probes.Close.Cytocell Ltd Oxford Gene Technology 418 Cambridge Science Park Milton Road Cambridge CB4 0PZ Phone +44(0) 1223 294048 Fax +44(0) 1223 294986 Email [email protected]; Twitter;20x SSC Buffer - Cytocell20x SSC Buffer.Applications ancillary items.Catalogue Numbers PCA 003.Request a quote.Request a sample.Unit Size 100ml.Browse all Probes.Close.Cytocell Ltd Oxford Gene Technology 418 Cambridge Science Park Milton Road Cambridge CB4 0PZ Phone +44(0) 1223 294048 Fax +44(0) 1223 294986 Email [email protected]; Twitter;

20X SSC Buffer IBI Scientific

Buffer containing Sodium chloride and Sodium citrate; SSC Buffer is a solution formulated for use in nucleic acid preparation and blotting applications,including northern blotting.SSC Buffer is used in concentrations ranging from 0.2X to 20X solutions depending on the application.20X SSC Buffer IBI ScientificBuffer containing Sodium chloride and Sodium citrate; SSC Buffer is a solution formulated for use in nucleic acid preparation and blotting applications,including northern blotting.SSC Buffer is used in concentrations ranging from 0.2X to 20X solutions depending on the application.(Discontinued) 20x SSC #1610774 Life Science Research 1 L,20x SSC,blot transfer and processing buffer,pH 7.0.This product is no longer available.Please contact technical support for more information on replacement product..View similar items Buffers and Reagents for Nucleic Acid Electrophoresis

results for this questionWhat is SSC buffer?What is SSC buffer?SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures.It is used to control stringency of wash buffer for the washing steps after hybridization.SSC Buffer 20 SSC 20X buffer#215; Concentrate for Northern and Southern blotting,solutio results for this questionWhat is 20X SSC solution?What is 20X SSC solution?The 20x SSC Solution is a 20x concentrate which needs to be diluted before use in in situ hybridization procedures (FISH or CISH) according to the needs of the user.20x SSC Solution 20x SSC Solution - results for this questionFeedbackSSC 20X Quality Biological

SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization.The concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5X.

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